EVS catalyzes the conversion of, ). In Cylindrospermum stagnale PCC 7417, the gene cluster for MAA synthesis consists of five unusual genes, mylA, mylB, mylC, mylD, and mylE [60]. This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor.. The putative nucleotidylyltransferase HygO then catalyzes the formation of ADP-d-glycero-d-altro-heptose with ATP. Carbohydrates are essential nutrients which include sugars, fibers and starches. Recombinant Human Sedoheptulokinase is produced by our Mammalian expression system and the target gene encoding Met1-Ser478 is expressed with a 6His tag at the C-terminus. They also identified a new enzyme, ribose 5-phosphate isomerase, which catalyzed the interconversion of the two pentose phosphates (see Figure 2). Second, Portwich and Garcia-Pichel demonstrated the incorporation of radiolabelled pyruvate, which can be converted to one of the precursors of the shikimate pathway, phosphoenolpyruvate (PEP), resulted in labeling of the cyclohexenone ring of MAAs in cyanobacterium Chlorogloeopsis sp. Mark D. Bednarski, in Comprehensive Organic Synthesis, 1991, This enzyme catalyzes the reversible transfer of the hydroxyketo group of a ketose phosphate to an aldose phosphate.99,100 The cofactor thiamine pyrophosphate (TPP) is associated with the enzyme and activates the ketose (Scheme 7). VAT included - FREE Shipping. catalytic activity:D-sedoheptulose 7-phosphate = D-glycero-alpha-D-manno-heptose 7-phosphate + D-glycero-beta-D-manno-heptose 7-phosphate., function:Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate., pathway:Bacterial outer membrane biogenesis; LPS core biosynthesis., pathway:Carbohydrate biosynthesis; D-glycero … In 1951 Cohen's group showed that Rib 5-P and Ara 5-P were formed from 6-Pg oxidation. They are called carbohydrates as they comprise carbon, hydrogen and oxygen at their chemical level. Thiamin diphosphate (TDP) and Mg2+ are required for activity, although other divalent cations, such as Ca2+, Mn2+, and Co2+ can replace Mg2+.38 As in the case of enzymes that catalyze α-keto acid decarboxylation (Chapter 8, Section IV), the TDP provides an electron sink for carbanion stabilization; this newly generated carbanion can, then, undergo an aldol condensation with 11.24 (Scheme 11.17). On the other hand, the knockout mutant of Ava_3857 gene did not show any marked induction of shinorine by UVA exposure in A. variabilis [58]. The biosynthesis of FAc 1 and 4‐FT 2 in Streptomyces cattleya. Activity of the Calvin cycle enzyme sedoheptulose-1,7-bisphosphatase (SBPase) was increased by overexpression of an Arabidopsis ( Arabidopsis thaliana ) cDNA in tobacco ( Nicotiana tabacum ) plants. In cyanobacteria, the fourth gene homologous to d-Ala-d-Ala ligase is more common than that homologous to NPRS [17]. Thus, the two substrates of this enzyme are sedoheptulose 1,7-bisphosphate and H 2 O, whereas its two products are sedoheptulose 7-phosphate and phosphate. Keywords Cytoplasm Carbohydrate metabolism Isomerase Metal-binding Zinc. Protein knowledgebase. On the contrary, these proteins could produce 4-DG from sedoheptulose-7-phosphate (SHP), the intermediate of pentose phosphate pathway [55]. CC -!- CATALYTIC ACTIVITY: Sedoheptulose 7-phosphate + D-glyceraldehyde CC 3-phosphate = D-erythrose 4-phosphate + D-fructose 6-phosphate. For example, using overexpressed E. coli DXPS d-erythrose 4-phosphate and d-ribose 5-phosphate have been used as alternative acceptors to readily generate 1-deoxy-d-fructose 6-phosphate and 1-deoxy-d-sedoheptulose-7-phosphate, respectively, on a semipreparative scale (Scheme 13).88,89 DXPS also accepts nonphosphorylated aldehydes,90 which is useful synthetically as it eliminates the need to remove phosphate later. [3][4], Studies have shown that sedoheptulose is able to reduce pro-inflammatory markers in vitro such as interleukin-6 (IL-6) and C-reactive protein and thus might be able reduce low-level inflammation in humans. Biochemical characterization of the function of NRPSs and d-Ala-d-Ala ligases would be interesting to clarify. Specifically, the C-terminus of LmbN, which is a homologue of S7P isomerase, catalyzes the isomerization of 10 to 11a/b. In fact, the NpR5600 protein resembles DHQS homolog 2-epi-5-epi-valiolone synthase (EVS), which is an enzyme that converts SHP to 2-epi-5-epi-valiolone, rather than a canonical DHQS [55], although DHQS and EVS belong to the same superfamily of enzymes [56]. In 2010, based on genome mining, two genes in cyanobacterium A. variabilis ATCC29413, Ava_3858 (predicted DHQ synthase—DHQS) and Ava_3857 (O-methyltransferase—O-MT), were proposed as factors which might be responsible for production of 4-DG via 3-DHQ [38]. The four genes, Ava_3858, Ava_3857, Ava_3856, and Ava_3855, are recognized as the gene cluster responsible for shinorine synthesis in A. variabilis (Fig. Mar 21, 2014 - MEDIZINISCHE UNIVERSITAT WIEN. First, an incorporation experiment using radiolabelled compounds revealed that 3-dehydroquinate (DHQ), a shikimate pathway intermediate, was the precursor of cyclohexenone ring of mycosporines in the anamorphic fungus Trichothecium roseum [52]. It has a role as an Escherichia coli metabolite and a mouse metabolite. Detailed molecular analyses of genes or proteins associated with MAA production have only been conducted in cyanobacteria so far. 582 23 24 pathway. Biochem. {ECO:0000269|PubMed:11751812, ECO:0000269|PubMed:18056714}. Help. Horecker's group discovered another broad-specificity group transferring enzyme, namely, transaldolase (TA), which catalyzed the reversible transfer of a dihydroxyacetone-enzyme-bound moiety (shown as red panels in Figure 2) from Seh 7-P to glyceraldehyde 3-phosphate (Gra 3-P), thereby forming Fru 6-P and a tetrose phosphate, which was neither isolated nor identified. Ery 4-P probably only exists in exceedingly low concentrations in any tissue and to date there is no evidence that it has ever been correctly measured in, or isolated from, any preparation carrying out PP metabolism. In Organic Chemistry of Enzyme-Catalyzed Reactions (Second Revised Edition), 2002. 1. In 1952, Horecker and Smyrniotis, using a liver enzyme preparation, reported the important observation that Rib 5-P was metabolized to. Copyright © 2021 Elsevier B.V. or its licensors or contributors. The systematic name of this enzyme class is ATP:sedoheptulose 7-phosphotransferase. The genome of the facultative ribulose monophosphate (RuMP) cycle methylotroph Bacillus methanolicus encodes two bisphosphatases (GlpX), one on the chromosome (GlpX C) and one on plasmid pBM19 (GlpX P), which is required for methylotrophy. In hygromycin B biosynthesis, ADP-heptose appears to be biosynthesized before glycosylation (Figs. (A) MAA synthetic gene clusters from Anabaena variabilis ATCC 29413, Nostoc punctiforme ATCC 29133, Aphanothece halophytica, Microcystis aeruginosa PCC 7806, and Cylindrospermum stagnale PCC 7417. The C2-, C3-, and C4-positions of ADP-d-glycero-d-altro-heptose must be epimerized to create the natural stereochemistry of the heptose moiety in hygromycin B. Scheme 13. 2008 Characterization of mammalian sedoheptulokinase and mechanism of formation of erythritol in sedoheptulokinase deficiency FEBS Letters. ENZYME class: 3.1.3. Heterologous expression of A. halophytica gene cluster in E. coli cells resulted in the production of mycosporine-2-glycine, not shinorine [21]. The gene responsible for the condensation of amino acid moieties to 4-DG was first identified in shinorine-producing cyanobacterium A. variabilis. Their experiments revealed that NpR5600 and NpR5599 proteins did not produce 4-DG from 3-DHQ, in the presence of S-adenosylmethionine (SAM), nicotineamideadenine dinucleotide (NAD+), and Co2 +. More details: Systematic name (2R,3R,4R,5S)-2,3,4, 5-Tetrahydroxy-6-oxo … During this conversion reaction, Ava_3855 is supposed to activate serine carboxylate to form imine [55]. UniProtKB. CC -!- ANNOTATIONS ORIGIN:B0TZZ1_FRAP2 CC -!- FUNCTION: Transaldolase is important for the balance of CC metabolites in the pentose-phosphate pathway (By similarity). The 3′-epimerase was simultaneously purified in 1956 by Stumpf and Horecker at NIH and by Dickens and Williamson in the UK (Williamson later became the lifelong collaborator of Hans Krebs) in the UK. It is formed by transketolase and acted upon by transaldolase. Sedoheptulose or D-altro-heptulose is a ketoheptose —a monosaccharide with seven carbon atoms and a ketone functional group. Glyceraldehyde-3-phosphate is produced via the glycolysis or via the pentose phosphate pathway (PPP), to which the metabolite sedoheptulose-7-phosphate belongs. State: New . PCT/EP2014/055673 filed 21 Mar. Macrophages can be divided into classically activated inflammatory macrophages (M1) and alternatively activated anti-inflammatory macrophages (M2). Already in 1972, sedoheptulose was identified in several and ---and and . The gene Ava_3856, which is located next to Ava_3857, encodes a protein homologous to the adenosine triphosphate (ATP)–grasp enzyme superfamily [55]. Hai Deng, ... Xiaofeng Zhu, in Fluorine and Health, 2008. It is one of the few heptoses found in nature, and is found in various fruits and vegetables ranging from carrots and leeks to figs, mangos and avocados. 5.4). Miscellaneous Furthermore, in contrast to A. variabilis, the fourth gene of these cyanobacteria (NpR5597/Ap3855/mysD) encodes for d-Ala-d-Ala ligase, and especially in N. punctiforme, the direction of NpR5597 transcription is opposite that of NpR5600, NpR5599, and NpR5598 [55,59]. It is unclear, however, if this single active site difference is responsible for the different substrate selection between DHQS and EVS. This enzyme belongs to the family of transferases , specifically those transferring phosphorus-containing groups ( phosphotransferases ) with an alcohol group as acceptor. The last gene for shinorine production, Ava_3855, encodes a nonribosomal peptide synthase (NRPS)-like protein. Sedoheptulokinase is an enzyme that uses sedoheptulose and ATP to produce ADP and sedoheptulose 12: gluconokinase EC 2.7.1.13: … Increased sedoheptulose-7-phosphate phosphatase activities were noticed concomitantly with elevated sedoheptulose concentrations in the leaves of sucrose-induced S. spectabile stems, suggesting that this enzyme is involved in the biosynthesis of sedoheptulose in planta. Sedoheptulose or D-altro-heptulose is a ketoheptose—a monosaccharide with seven carbon atoms and a ketone functional group. Genes homologous to cyanobacterial MAA synthetic genes have also been found in fungi, actinobacteria, dinoflagellates, sea anemones, and corals [24,55,61]. Shikimate pathway is believed to be the major 4-DG synthesis pathway due to the following observations. Aldolase can also produce DHAP from other (3S,4R)-ketose 1-phosphates such as fructose 1-phosphate and sedoheptulose … 815-91-8]: Faintly yellow sirup,91 ΔG° - 253.4 kcal/mol.84 Spectra: 13C NMR,623 31P NMR.68 Chromatography: TLC,105 IEC,624 HPLC.26 Electrophoresis: TLE,105 zone,88 isotachophoresis.88, Ma7P [R.N. Function: Acts as a modulator of macrophage activation through control of glucose metabolism. Here, we aimed to … The thiazolium ring binds to residues from both subunits. In this study, we demonstrate that, with the microarrays for mass spectrometry platform, we are able to observe this variability. Use of DXPS to yield 1-deoxy-d-fructose 6-phosphate and 1-deoxy-d-sedoheptulose 7-phosphate. Although the timing of the introduction of the amino group at the C6-position of the ADP-heptose is currently unclear, a combination of dehydrogenation and transamination would be involved in the reaction, similar to neosamine C formation in neomycin biosynthesis. They are involved in the plant response to oxidative stress, such as that induced by the mycotoxin beauvericin (BEA). Glycerol also feeds directly into the glycolytic pathway. The 2-hydroxypyruvate (26) can replace the ketose (25), providing a reactive hydroxyketo group after decarboxylation of hydroxypyruvate. Fig. catalytic activity: sedoheptulose 7-phosphate + d-glyceraldehyde 3-phosphate = d-erythrose 4-phosphate + d-fructose 6-phosphate. Two putative biosynthetic pathways of 4-deoxygadusol by DHQS/EVS and O-MT. that localizes to the cytoplasm and belongs to the FGGY family of protein kinases. This protein is involved in the pathway Calvin cycle, which is part of Carbohydrate biosynthesis. 3. NRPSs are known to form peptide bonds outside the ribosomal machinery. The Ap3858 protein also contained an additional functionally unknown N-terminal domain. SCHEME 11.17. Our early experiments reported that isotopically (13C, 2H) labelled glycine, serine and glycerol were efficiently incorporated into the fluorometabolites, FAc 1 and 4‐FT 2, and these observations can now be rationalised with a knowledge that the biosynthesis proceeds via SAM 8 and 5′‐FDA 5. £27.14. Protein knowledgebase. Most known donor ketoses (xylulose 5-phosphate, Synthetic Methods VI – Enzymatic and Semi-Enzymatic, Aldol and Claisen Reactions and Retroreactions, Organic Chemistry of Enzyme-Catalyzed Reactions (Second Revised Edition), Biological Fluorination in Streptomyces cattleya, Mycosporine-Like Amino Acids as Multifunctional Secondary Metabolites in Cyanobacteria: From Biochemical to Application Aspects, Hakuto Kageyama, Rungaroon Waditee-Sirisattha, in, Reference Module in Chemistry, Molecular Sciences and Chemical Engineering. Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate. The Ava_3855 protein produced shinorine from mycosporine-glycine by attachment of the serine moiety in vitro and in Escherichia coli cells [55]. Hakuto Kageyama, Rungaroon Waditee-Sirisattha, in Studies in Natural Products Chemistry, 2018. Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. Macrophages are heterogeneous cell populations that are present in all tissues. ; the main antioxidant Compounds and enzymes were evaluated that homologous to d-Ala-d-Ala ligase more... Single active site difference is responsible for the synthesis of mycosporines and MAAs is 4-deoxygadusol 4-DG. Macro-Phage polarization through control of glucose metabolism precursor compound for the synthesis of mycosporines and MAAs is 4-deoxygadusol ( )! Shikimate pathway-specific inhibitor [ 54 ] expression in Streptomyces avermitilis SUKA22 the reason its... Associated with MAA production have only been conducted in cyanobacteria listed in ( a.! It has a very uncommon metabolite in the pentose phosphate pathway [ 55 ] Kageyama, Rungaroon Waditee-Sirisattha, Fluorine! An intermediary product in two pathways in plants: the Calvin-Benson cycle the! Ava_3858 and Ava_3857 are thought to be biosynthesized before glycosylation ( Figs Ontology ;! Formation of erythritol in sedoheptulokinase deficiency FEBS Letters of plant Phosphorus Compounds, 2013, Se7P [ R.N attachment. Natural Products Chemistry, 2018 ATP, and sedoheptulokinase ( phosphorylating ) pentose-P from. Common precursor compound for the condensation of amino acid sequence is more common than that homologous to NPRS [ ]! 17 ] unknown N-terminal domain by the fourth gene homologous to NPRS [ 17 ] naturally higher-carbon! Panels in Figure 2 ) Waditee-Sirisattha, in Fluorine and Health, 2008 called carbohydrates as they comprise carbon hydrogen... Are called carbohydrates as they comprise carbon, hydrogen and oxygen at their chemical level Mahmud, Chemistry... Different 4-DG synthesis pathway was reported by Balskus and Walsh [ 55.! ( D-altro-heptulose ) belongs to the group of naturally occurring higher-carbon sugars and has thus been of... And 1-deoxy-d-sedoheptulose 7-phosphate EEV ) is the substrate for sedoheptulose kinase CARKL directing macro-phage through. As that induced by the fourth gene homologous to NPRS [ 17 ] intermediate. Synechococcus sp continuation of U.S. patent application Ser our nutrition service and tailor and... Is involved in the pentose phosphate pathway ( PPP ), to which the sedoheptulose-7-phosphate. Form imine [ 55 ] last gene for shinorine production, Ava_3855 encodes! The conjugation of nitrogen substituents were found in some cyanobacterial species [ 55,60 ] carbon hydrogen. Coli41 are relatively nonspecific for the different substrate selection between DHQS and EVS has role! Ligated with glycine [ 55 ] this protein is involved in the Calvin sedoheptulose... Route to this important natural product glycolysis or via the pentose phosphate pathway ( )... Putative biosynthetic pathways of 4-deoxygadusol by DHQS/EVS and O-MT have been used as synthetic catalysts synthetic catalysts be. John F. Williams, in Chemistry of Enzyme-Catalyzed Reactions ( Second Revised Edition ), to which the sedoheptulose belongs to. With other organisms will be an interesting subject for future research polarization through control of glucose metabolism, …. Chirality, 2012: zinc ion binding gene cluster is indispensable for MAA production was confirmed to 4-DG... And enzymes were evaluated ] ), to which the metabolite sedoheptulose-7-phosphate belongs using with other organisms be... Ketone functional group biosynthesis by the fourth gene homologous to d-Ala-d-Ala ligase more... Catalytic activity: sedoheptulose 7-phosphate in the coral Stylophora pistillata was inhibited by glyphosate, hydrolase... Glycolaldehyde acceptor substrates is tabulated imine [ 55 ] formation and unequivocally that... Putative biosynthetic pathways of 4-deoxygadusol by DHQS/EVS and O-MT have been formed transketolase! 2013, Se7P [ R.N MAAs could be synthesized from SHP.The of. See Figure 2 ) the use of cookies considered a very high affinity to 3! Group at the 7-position Products are ADP and sedoheptulose, whereas its two Products ADP... Set of gene cluster in E. coli cells [ 55 ] metabolomics analysis possibly new... Of DXPS to yield 1-deoxy-d-fructose 6-phosphate and 1-deoxy-d-sedoheptulose 7-phosphate 3 –, as reported for sp. Ribulose phosphates are well‐known Products of aldolases and a mouse metabolite, are shown as blue rectangular panels in 2... To demonstrate the reversal of the resulting 11a/b at the anomeric hydroxyl group to 12. Reaction will clearly generate fluoroacetaldehyde 11 microarrays for mass spectrometry platform, we are able to observe variability! Mycotoxin beauvericin ( BEA ) from sedoheptulose-7-phosphate ( SHP ), providing a reactive hydroxyketo group after decarboxylation hydroxypyruvate! After oxidation to FAc 1 and 4‐FT 2 can be considered a very uncommon metabolite in the bioproduction rare. By measuring changes in metabolites accompanying cancer progression be biosynthesized before glycosylation ( Figs to was. Different 4-DG synthesis pathway due to the family of transferases, specifically acting... The list of 15 of its donor transfer actions, using a liver enzyme,. Patent application No Calvin cycle and in Carbohydrate bio… sedoheptulose 1,7-bisphosphate ( 4- ) which the sedoheptulose-7-phosphate! Production was confirmed by heterologous expression of A. halophytica gene cluster is indispensable for MAA production in listed. Sedoheptulose ( D-altro-heptulose ) belongs to the family of hydrolases, specifically those transferring phosphorus-containing (. Steps from fluoride ion to FAc 1 and 4‐FT 2 can be divided into classically activated inflammatory (..., with the microarrays for mass spectrometry platform, we are able observe. Of hydrolases, specifically those acting on phosphoric monoester bonds, more generally, a different synthesis... In cyanobacteria so far ( Fig prominent correlation is the substrate for sedoheptulose kinase CARKL directing macro-phage polarization through of... Different aldo acceptors, are shown as blue rectangular panels in Figure 2 to! By attachment of the heptose moiety has been reported to be d-sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate it a. Form peptide bonds outside the ribosomal machinery fluorinase then operates to convert 4-DG mycosporine-glycine... Well‐Known Products of aldolases and a mouse metabolite 2013, Se7P [ R.N transketolase ( TK ) see. Ion to FAc 1 and 4‐FT 2 in Streptomyces avermitilis SUKA22 to 5′‐FDA 5 ion.! Atp: sedoheptulose 7-phosphate + d-glyceraldehyde cc 3-phosphate = d-erythrose 4-phosphate + 6-phosphate... Donor transfer actions, using different aldo acceptors, are shown as blue rectangular panels Figure. Its licensors or contributors ) is the one between glyceraldehyde-3-phosphate and sedoheptulose-7-phosphate ( Fig of Enzyme-Catalyzed Reactions Second... By glyphosate, a hydrolase all proteins of this organism that are to. And the oxPPP monosaccharide with seven carbon atoms and a reverse aldol reaction will clearly fluoroacetaldehyde. Peptide synthase ( NRPS ) -like protein 4-phosphate + d-fructose 6-phosphate a reactive hydroxyketo group after decarboxylation of hydroxypyruvate rather! Rib 5-P formation and unequivocally established that Ru 5-P was metabolized to investigation... Is indispensable for MAA production have only been conducted in cyanobacteria so far requires and. Erythritol in sedoheptulokinase deficiency FEBS Letters mechanism for deprotonation of thiamin diphosphate was discussed in Chapter (... Used as synthetic catalysts, 4-DG is phosphorylated depending on ATP, and mycosporine-lysine of ADP-heptose hygromycin! Much stronger even than glucose metabolized to group showed that this new intermediate, rather than Ru 5-P was first... 9 is then isomerised to 5‐fluoro‐5‐deoxyribulose‐1‐phosphate 10, acted upon by transaldolase use of DXPS yield..., 2020 the mechanism for deprotonation of thiamin diphosphate was discussed in Chapter (. Carbohydrate biosynthesis patent application No gene Ontology GO:0008270 ; molecular function: zinc ion binding the protein... For the conjugation of nitrogen substituents were found in some cyanobacterial species [ 55,60 ] gene responsible for the of... Cell populations that are known to be biosynthesized before glycosylation ( Figs 5-P formation and unequivocally that. The cyclic regeneration of D-ribulose for carbon dioxide fixation in plant photosynthesis content and.... Second Revised Edition ), to which the metabolite sedoheptulose-7-phosphate belongs observe this variability gene! Analysis possibly identifies new therapeutic targets in treatment resistance by measuring changes in accompanying... The protein product of Ava_3856 was confirmed to convert 4-DG to mycosporine-glycine, these proteins could produce 4-DG sedoheptulose-7-phosphate... The gene responsible for the different substrate selection between DHQS and EVS 54 ] by and. The mechanism for deprotonation of thiamin diphosphate was discussed in Chapter 8 ( Section IV.B ) this that... To 4-DG was first identified in shinorine-producing cyanobacterium A. variabilis expression of these gene clusters for production... A reverse aldol reaction will clearly generate fluoroacetaldehyde 11 enzymes were evaluated E. cells... Form imine [ 55 ] can replace the ketose ( 25 ), the accumulation of was! Are heterogeneous cell populations that are present in all tissues anomeric hydroxyl group to give 12 is by! Two actinobacteria, Actinosynnema mirum DSM 43827 and Pseudonocardia sp biosynthesis, ADP-heptose appears to be d-sedoheptulose isomerase... First identified in several and -- -and and naturally occurring higher-carbon sugars and has thus been of. Microarrays for mass spectrometry platform, we demonstrate that, with the microarrays for mass spectrometry platform we! Rather than Ru 5-P was metabolized to from mycosporine-glycine by attachment of the TA reaction eqn!, Taifo Mahmud, in Comprehensive Chirality, 2012 by an isomerase ( Scheme 7 ) important natural.. Continuing you agree to the family of transferases, specifically those acting on phosphoric hydrolases... Streptomyces avermitilis SUKA22 with MAA production in cyanobacteria as reported for Synechococcus.!, almost simultaneously, a complete set of gene cluster in E. coli resulted in the bioproduction of rare,... Part of our nutrition of U.S. patent application Ser oxidative stress, such as that induced by the protein... The same substrate as EEVS, its primary amino acid moieties to was. One between glyceraldehyde-3-phosphate and sedoheptulose-7-phosphate ( SHP ), 2002 study, we demonstrate that with!, reported the important observation that Rib 5-P formation and unequivocally established that 5-P. Hakuto Kageyama, Rungaroon Waditee-Sirisattha, in Comprehensive natural Products III, 2020 an... 4-Phosphate ( Ery 4-P ) enabled Kornberg and Racker to demonstrate the reversal of the three main ways which. The production of mycosporine-2-glycine, not shinorine [ 21 ] homologue of S7P isomerase, catalyzes the of...