Preparation of blood smear. You must push more slowly to ensure that the blood spreads far enough down the slide. With the advent of hematological automated machines, complete and highly accurate hematological data and parameters like complete blood count or CBC can be obtained within a few minutes. Rouleaux formation is a striking feature that results from a high level of serum M-protein. A small drop of blood is placed on the midline at the end of a glass slide. Meeting these parameters gives you the best opportunity to see cells as they would be in vivo and approximately quantify them. A high hematocrit level leads to thicker blood; a low hematocrit means thinner blood. 1) On one slide (the sample slide), place a small drop of blood . The healthcare professional will collect a small amount of blood in a test tube using a needle inserted into one of the veins in the patient’s arm. Push the edge of the pusher slide into the blood drop so that it spreads out completely along the pusher slide. endstream <> However, the effectiveness and accuracy of that interpretation depend on the quality of the slide. Textbooks teach you to place the patient slide on the table and push the pusher slide, with the shorter edge touching the blood, away from you. 2 0 obj endobj You can also change the angle of the pusher slide. High hematocrits occur most frequently in newborns and patients with certain cancers such as polycythemia vera. 6. Optimise spreading speed for length and a good feathered edge. Left to right: • slide 1 – perfect smear • slide 2 – smear technique interrupted in middle • slide 3 – smear was skewed • slide 4 – blood droplet too thick • slide 5 – smear … Appearance of WBC's nuclei in a well-stained peripheral blood smear microscopically. Types of thin film technique. Maintain even contact throughout the spreading motion. The three main blood cells that the test focuses on are: red cells, which carry oxygen throughout your body Aim for a blood droplet size of 4mm diameter. Keep practicing with these guidelines in mind, and you will be making textbook-perfect slides in no time. When making a wedge smear, there are two slides involved: the pusher slide and the patient slide. The blood smear test plays an important role in the speedy diagnosis of certain infections or diseases. Techniques of making good bacterial smear. Slide method 2. stream There are many reasons why your doctor may order a blood smear. 6 0 obj %���� When done correctly, it should result in a uniform blood film, that gets progressively thinner. Push the blood down the patient slide with the pusher slide. The quality of the blood smear depends on a proper technique and quality of the staining. Slide three? If … The purpose of preparation of blood smear (blood film} is to study the morphology of RBCs, differential leukocyte count and reticulocyte count. This site uses Akismet to reduce spam. <>/XObject<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 720 540] /Contents 13 0 R/Group<>/Tabs/S/StructParents 1>> Using a capillary tube collects blood from the tube and put two large drops at the center of a sterile microscopic slide. Copyright © 2020 Science Squared - all rights reserved, Analytical Chemistry and Chromatography Techniques. A blood smear involves looking at a sample of blood under the microscope after applying special stains and looking for abnormalities or changes in red blood cells, white blood cells, and platelets. Name the types of peripheral blood smear. Giemsa stain is commonly used when there is need to examine the Blood smear for the Parasites but is a good stain for routine examination of blood smear and used to differentiate nuclear and cytoplasmic morphology of the various cells of the blood like Platelets, RBCs.... Giemsa Staining Technique… Thin smear; Thick smear; What are techniques for peripheral smear preparation. <> One of the most common types of peripheral blood slides is the wedge slide. Slide technique; Cover slip technique; Automated slide making and staining; What is the procedure of preparing peripheral smear ? Ten slides later and you still don’t have a fantastic, easy to read slide. endstream endobj 5 0 obj Hello, Friends ... Leishman Stain Procedure,ideal blood smear,blood smear technique. PDF | The process of medical diagnosis is an important stage in the study of human health. They can be a useful tool to assess everything from white blood cell disorders, such as leukemias, to various types of acquired or congenital anemias, to broader organ issues like liver or kidney disease. endstream If you have any other tips or insights on making peripheral blood smears, feel free to put them in the comments! <> Blood smear: slide-to-slide technique This item is available to registered subscribers only Sign up now to obtain ten tokens to view any ten Vetlexicon articles, images, sounds or videos, or Login Posted in Education. stream An ideal slide is neither too thin nor too thick. When making a wedge smear, there are two slides involved: the pusher slide and the patient slide. There are many ways to hold the slides, depending on what works best for you. Place either the long or the short edge of the pusher slide just in front of the blood drop, holding it at an approximately 30-degree angle from the patient slide. The technique for making and storing dried blood samples is given in the section “Dried Blood Samples”. Most starting external quality o … When I make a slide, I have confidence that it will be a quality smear, which helps me maintain a consistent speed as I push. Normally, the pusher slide is held at a 30-degree angle from the patient slide, but it may be necessary to decrease that angle for patients with high hematocrits to prevent the smear from being too short and thick. APPARATUS: 4-5 glass slides, compound microscope, pricking needle (blood lancet), spirit swab, cedar wood oil/liquid paraffin, Leishman’s stain, wash bottle, buffered water and staining tray. 13 0 obj As with everything, practice makes perfect. endobj endobj Use clean, high-quality microscope slides. When I make a slide, I know what adjustments to make as soon as I see the blood spread against the pusher slide. 2) Use the second slide as a “spreader” slide. In the end, all that matters is that the pusher slide edge is flat against the patient slide. A blood smear evaluates red blood cells (RBC morphology) white blood cells (WBC) and platelets to help diagnose anemia, infections or blood disorders. Thin blood spreads more quickly, you need to make as soon as I the... Ways to hold the spreader slide at 30-40 degrees to achieve optimal smear length when done correctly, should... And approximately quantify them one slide ( the sample slide ), place a small drop of blood and! Rights reserved, Analytical Chemistry and Chromatography techniques blood in an EDTA tube make. Interpretation depend on the left is too big and will result in a uniform blood film, gets! A proper technique and quality of peripheral blood smear microscopically level leads to thicker blood ; a low means! Far down the patient ’ s age and health status can help get enough to. 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