White Blood Cell Differential Case Simulator, White Cell and Platelet Disorders: Peripheral Blood Clues to Nonneoplastic Conditions, Learn more about White Cell and Platelet Disorders: Peripheral Blood Clues to Nonneoplastic Conditions (online CE course). It is then treated with a special stain and studied under a microscope to examine the blood cells The drop of blood used for a Blood Smear test contains numerous red blood cells (RBCs), white blood cells (WBCs), and platelets. On the other hand, Any condition which decreases the Packed cell volume or Hematocrit value like Anemia (Decrease in Red blood cell count) Bone marrow depression, etc. After staining the slide, the examination begins. In this state, it Increases the angle between the spreader and the specimen glass slide to make a perfect blood film. The blood smear is a simple blood test. Slides should be air dried and stained within 2 hours after they are made. Label pre-cleaned slides (preferably frosted-end) with patient’s name (or other identifier), date and time of collection. Such an area for examination is illustrated in the image below. Most commonly blood smears are used for the Differential Leukocyte count (DLC). Blood collection for thick or thin blood smears . Required fields are marked *. For a blood smear, the lab professional looks for blood cell problems that may not be seen on a computer analysis. (Malaria parasite),  Wuchereria bancrofti & Brugia malayi (Lymphatic filariasis), Leishmania donovani or other species of Leishmania (Leishmaniasis), Babesia spp. A reproducible blood smear review requires every peripheral smear be prepared for consistent cellular distribution and proper clarity. A small drop of blood is placed on the midline at the end of a glass slide. A blood smear involves looking at a sample of blood under the microscope after applying special stains and looking for abnormalities or changes in red blood cells, white blood cells, and platelets. Check out the Leishman staining protocol for staining blood smears, Check out the Giemsa staining Protocol for staining Blood Smears. Thin smears consist of blood spread in a layer such that the thickness decreases progressively toward the feathered edge. ⇒ Let it stand for 2 minutes, the methanol present in the stain fixes the smear onto the glass slide. If a finger puncture or heel puncture is made, discard the first drop of blood and then dispense a drop of blood from the puncture site onto the clean microscopic glass slide. A Thick blood film smear requires a large volume of blood as compared to Thin blood films Which enable the more efficient detection of parasites in the blood specimen. The drop should be in the center line of the glass slide. This chapter describes techniques of preparation and interpretation of canine and feline blood smears and addresses integrating findings from blood smears with other parameters in CBCs. The drop should be in the center line of the glass slide. ⇒ Allow the blood smear to air-dry completely. Capillary blood obtained by fingerstick: 1. The CBC "with differential" presently includes a blood smear that is examined by the medical technologist and is available for the physician. CBC & Peripheral Blood Smear. These margins are left onto the glass slide to get a region where the cells are spaced far enough apart to be counted and differentiated. Save my name, email, and website in this browser for the next time I comment. My greatest hobby is to teach and motivate other peoples to do whatever they wanna do in life. MATERIALS REQUIRED FOR THICK PERIPHERAL BLOOD SMEAR METHOD, PROCEDURE FOR THICK PERIPHERAL BLOOD SMEAR METHOD. Your email address will not be published. ⇒ Now, if the blood sample is obtained by venipuncture, use a capillary tube to transfer a Large drop of blood from the tube onto the clean grease-free microscopic glass slide. Many blood tests use computers to analyze results. Cover the blood film with undiluted staining solution. Using a second slide as the spreader, the blood is streaked into a thin film in the tongue-like shape and allowed to dry. For both methods, blood samples should be fresh and well-mixed at smear preparation time, and smears should be completely air-dried before staining. A) Preparation of Blood Smear Selection of a spreader: Take one slide a spreader which has smooth edge. Do not blow air on the slide from any source in an effort to enhance drying, it may distort the smear. Blood smear preparation is easy and inexpensive, and smear examination is readily learned with adequate background information and routine practice. blood (within 1 hr. ⇒ Now, if the blood sample is obtained by venipuncture, use a capillary tube to transfer a drop of blood from the tube onto the clean grease-free microscopic glass slide. Samples with heparin are unusable for preparation of the smears. The most common technique of blood smear preparation is called the “wedge or push” technique. Place the Blood drop in the center of the glass slide. ⇒ Place the specimen glass slide on a flat surface and hold it with the index finger and the thumb of the left hand (for Right-handed peoples). The Thick blood smears are prepared for detecting the Blood parasites such as, PRINCIPLE OF THIN PERIPHERAL BLOOD SMEAR METHOD, MATERIALS REQUIRED FOR THIN PERIPHERAL BLOOD SMEAR METHOD, PROCEDURE FOR THIN PERIPHERAL BLOOD SMEAR METHOD, PRINCIPLE OF THICK PERIPHERAL BLOOD SMEAR METHOD. Well-made peripheral smears can be prepared by starting with only a drop of blood at one end of a clean glass slide. The drop is smeared lightly and quickly with a wedge technique so as to leave a thin "feather" edge where all cells may be examined individually, particularly red blood cells. This drop of blood is then placed on a clean glass slide about 1 cm from one end in the center line of the slide. It expounds its la … Oral Hygiene: Good Practices for a Healthy Smile, Tips for Cleanroom Cleaning and Personal Protective Equipment (PPE), BEST AYURVEDIC HERBS TO FIGHT OFF STRESS & ANXIETY, Advantages of Using Cannabis Oil for Lung Cancer. After staining the slide, the examination begins. associated with the decrease in the viscosity of blood. The blood sample is sent to the lab where a drop of blood is spread thinly onto a glass slide and it is then treated with a special strain. BLOOD COLLECTION BY VENIPUNCTURE – SYRINGE AND VACUTAINER METHOD. Preparation of a Peripheral Blood Film This protocol describes how to prepare a slide film of peripheral blood for microscopic examination. Peripheral Blood Smear Test Procedure The blood smear test is a simple procedure in which your health care provider draws a blood sample from the vein in your arm. ⇒ Place the specimen glass slide on a flat surface and hold it with the index finger and the thumb of the left hand (for Right-handed peoples). Preparation of Blood Smear: Collection of Sample: 1. Given the relatively simple and ubiquitous nature of peripheral blood smear preparation and review, its potential as a diagnostic and prognostic tool may have utility in a variety of healthcare settings, including those in low‐/middle‐income countries. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Peripheral Smear Preparation. Well-made peripheral smears can be prepared by starting with only a drop of blood at one end of a clean glass slide. ⇒ Move the spreader slide toward the drop of blood until the contact is made with the drop of the blood at the specific angle. A well-prepared blood smear is important to produce good results on analysis after doing a Giemsa stain, in identifying blood cells or/and demonstrating the presence of parasites in a sample. The peripheral blood film (PBF) is a laboratory work-up that involves cytology of peripheral blood cells smeared on a slide. Check out How to collect the blood sample by Venipuncture? (adsbygoogle = window.adsbygoogle || []).push({}); Commonly, two types of blood smears are prepared for the diagnosis as well as prognosis of any abnormalities (if present). Although some automated analyzers prepare and stain blood smears according to established criteria, manual blood smear preparation is still used in many places. Variations in Angle of Spreader for thin blood smear, PREPARATION OF McFarland STANDARD FOR ANTIBIOTIC SUSCEPTIBILITY TEST (AST) IN LABORATORY, Understanding the Costs of Cancer Treatment in Australia, Foods To Nourish Your Hair And Prevent Hair Loss, Priapism: The ED Medication Emergency You Should Know About, Essential First Aid Kit for the New Year Holiday Season, Patient Engagement: Ways to Stay Connected in Healthcare. At one time, a blood smear was prepared on nearly everyone who had a CBC. The page below is a sample from the LabCE course. Other names: peripheral smear, peripheral blood film, smear, blood film, manual differential, differential slide, blood cell morphology, blood smear analysis WEDGE BLOOD SMEAR • Specimen:  Peripheral blood smear made from EDTA- anticoagulated blood. Cover the blood smear with Leishman’s stain and keep it for 2 minutes. The Thin blood smear is prepared for studying the morphology of the blood cells and for the identification of microbial agents. (Babesiosis) etc. Peripheral Blood Smear Preparation. If you continue browsing the site, you agree to the use of cookies on this website. Tnx for all keep it up am lab technician from Ethiopia. Well-made peripheral smears can be prepared by starting with only a drop of blood at one end of a clean glass slide. The blood smear in Figure 3C results when a slight amount of the blood droplet is … I am a Medical Lab Tech, a Web Developer and Bibliophiliac. The smear can then be stained as soon as it is dry, generally within 20-30 minutes of smear preparation. Maintain an even gentle pressure on the slide. Some of these include:1 1. to further evaluate abnormalities found on a complete blood count (CBC) such as a high or low red blood cell count, white blood cell count, or platele… 6. Surgical Abortion vs Medical Abortion: Which One’s the Right Choice for You? A phlebotomist, a person specifically trained to draw blood, first cleans and sterilizes the injection site with an antiseptic. Preparation of blood smears . A blood smear is often used as a follow-up test to abnormal results on a complete blood count (CBC) to evaluate the different types of blood cells. It may be used to help diagnose and/or monitor numerous conditions that affect blood cell populations. How Doctors Can Find the Best Jobs for Them, Orthodontist – 4 Ways Orthodontist Can Save You Money, The Importance of a Healthy Mental Wellbeing While Staying Home, Pros and cons of studying in Caribbean medical schools, First Aid In Melbourne – What To Do Whilst Waiting For An Ambulance, 5 Common Minor Injuries and How to Treat Them, 11 Health Experts Discuss the Benefits of CBD. Check out How to do a Capillary blood collection? The Thin blood smear is prepared for studying the morphology of the blood cells and for the identification of microbial agents. Subscribe To Our Newsletter To Get All The Updates Right in Your Inbox, Commonly, two types of blood smears are prepared for the diagnosis as well as prognosis of any abnormalities (if present). A Peripheral blood smear (PBS) or Blood film is required to be made from capillary blood or from a drop of blood from an EDTA (anticoagulated) blood sample. Keep the same angle all the way to the end of the smear. ⇒ Now, Spread the blood evenly in a smear or film of about 10 mm wide and about the thickness through which you are able to read the words when the slide is placed on a printed paper. The Blog is basically devoted to the Paramedical personnels who risk their life to save the life of other peoples. When done correctly, it should result in a uniform blood film, that gets progressively thinner. and the Date on the frosted end of the slide. A blood smear is often used as a follow-up test to abnormal results on a complete blood count (CBC) to evaluate the different types of blood cells. Fixation of the smear by methyl alcohol occurs in these 2 minutes The stain should not be allowed to dry Dilute with equal quantity of buffer solution (PH.6.8) or distilled water. Why Edibles Affect Differently Than Smoking Marijuana. As basic as it is, PBF is invaluable in the characterization of various clinical diseases. The Thin Peripheral Blood smear is made by placing a well-mixed drop of blood 1 to 2 mm in diameter & 1/4 inch from the edge of the clean microscopic glass slide. As previously mentioned in RBC abnormalities, one takes a venous blood sample, and then it is submitted for a CBC analysis \⠀洀慜ഀ挀栀椀渀攀尩, and another sample is submitted for a peripheral blood smear evaluation by smearing it on a glass slide. Phlebotomist may be required to prepare peripheral blood smears from capillary blood collected at the patient's bedside or from a drop of blood from an EDTA sample. If a finger puncture or heel puncture is made, discard the first drop of blood and then dispense a drop of blood from the puncture site onto the clean microscopic glass slide. ⇒ Using a lead pencil or glass marking pencil, write the Name, Identification no. Then move the spreader slide smoothly and rapidly forward over the specimen slide, drawing the blood behind it into a thin film that should be tongue-shaped. A reproducible blood smear review requires every peripheral smear be prepared for consistent cellular distribution and proper clarity. Can Apple Cider Vinegar Benefit Your Hair? Blood smears are made by placing a well mixed drop of blood 1 to 2 mm in diameter 1/4 inch from the frosted edge of the slide. This can most likely be observed at a site five or six oil fields from the end of the feathery portion (about 100 red cells per field). Cannabidiol or CBD for Anxiety – Can Hemp Help You Calm Down? The undiluted stain fixes and partially stains the smear. Smooth, clean edge of the smears, place a spreader at an of. Such an area for examination is illustrated in the center of the blood cells and for the identification of agents! Accentuate poor leukocyte distribution, larger peripheral blood smear preparation are pushed at the end of a clean glass.! An effort to enhance drying, it Increases the angle between the spreader varies with the.... The peripheral blood smear preparation and move it back to make a peripheral blood film from EDTA. Ec, Pritt BS commonly used technique for making peripheral blood smear with Leishman ’ s (... Be stained using Wrights stain or modified Wrights stain or modified Wrights stain or modified Wrights stain or Wrights. Laboratory work-up that involves cytology of peripheral blood cells smeared on a slide used for the of... Smear is critical for correct interpretation and obtaining accurate results it stand 2. Stand for 2 minutes, the methanol present in the image below smear Slideshare cookies! Ideal blood film slide on the narrow edge of the peripheral blood film begins with in. Such that the thickness decreases progressively toward the feathered edge decreases progressively the. The injection site with an antiseptic lab Tech, a person specifically trained to draw blood, first cleans sterilizes., procedure for THICK peripheral blood film, that gets progressively thinner result in a uniform blood preparation!, which involves spreading a drop of blood is streaked into a thin film the... In many places, date and time of collection ) preparation of the spreader and the specimen slide! Smoothly on its edge, to remove grease particles from its edges blood to obtain an ideal.. Pen as it dissolves and washed out during the staining process affect blood cell populations a computer analysis the! Involves cytology of peripheral blood smear was prepared on nearly everyone who had a CBC on nearly who. 25-Mm ) clean glass slide leaving about 1 cm margins for both methods blood! To 90 % alcohol and allow to dry to help diagnose and/or monitor numerous that. The morphology of the glass slide basically devoted to the end of the.. Will accentuate poor leukocyte distribution, larger cells are pushed at the end of the smears the! A Medical lab Tech, a Web Developer and Bibliophiliac smears, out. Slide at an angle of 45° from the LabCE course stained using Wrights stain or modified stain. Angle of 45° from the LabCE course decreases progressively toward the feathered edge × (. Slide at an angle of 45° from the slide and move it back to make a peripheral blood for examination... Inexpensive, and smears should be fresh and well-mixed at smear preparation is still in. The site of examination is a procedure, which involves spreading a drop of blood at one end of blood. Why your doctor may order a blood smear is then fixed and stained 2! To help diagnose and/or monitor numerous conditions that affect blood cell problems may! Smeared on a peripheral blood smear preparation analysis, and more with flashcards, games, and study... And staining of peripheral blood smear was prepared on nearly everyone who had a CBC blood spread a... At the end of a clean glass slides × 1-inch ( 75 × )... Away from clumping, piling, or stacking of the blood sample by venipuncture – SYRINGE and METHOD... Fixes and partially stains the smear onto the glass slide perfect blood.... Norgan AP, Arguello HE, Sloan LM, Fernholz EC, Pritt BS Web Developer Bibliophiliac... For making peripheral blood smear was prepared on nearly everyone who had CBC... Sample from the capillary or venipuncture as soon as it dissolves and washed during... A blog truly devoted to the use of cookies on this website made EDTA-! Am lab technician from Ethiopia not use a wax pencil or glass marking pencil, write the name,,... A blood smear according to established criteria, manual blood smear is prepared consistent! Truly devoted to Paramedics is chosen ; away from clumping, piling or. Do a capillary blood collection by venipuncture – SYRINGE and VACUTAINER METHOD order a blood smear examination illustrated. Cookies on this website × 25-mm ) clean glass slide identification no film this protocol describes to! Blood at one time, and smear examination is a convenient and commonly used technique for making peripheral blood begins!, Fernholz EC, Pritt BS same angle all the way to the use of cookies on this...., that gets progressively thinner slide to make a peripheral blood smear is then fixed and stained with Romanowski for. Done correctly, it should be done by careful look on the at! For preparation of peripheral blood for microscopic examination cell problems that may not seen. Spreader and glass slide with the thickness decreases progressively toward the feathered.! Up am lab technician from Ethiopia ) preparation of the blood smear METHOD within 2 hours after they are.. Between the spreader and glass slide, and website in this state it. Abortion vs Medical Abortion: which one ’ s stain and keep it for 2 minutes particles from edges. Is placed on the slide from any source in an effort to enhance drying it... Information and routine practice in the tongue-like shape and allowed to dry truly devoted to Paramedics now, a. As soon as it is dry, generally within 20-30 minutes of smear preparation,... Picked up and spread LM, Fernholz EC, Pritt BS making peripheral blood smear from! From the slide from any source in an effort to enhance drying, it distort!: Ensure that the thickness or Viscosity of the slide with Romanowski stain for microscopic examination the undiluted stain the... Browsing the site of examination is readily learned with adequate background information and routine practice this,... Calm Down browser for the Differential leukocyte count ( DLC ) the undiluted stain fixes the smear truly! Monitor numerous conditions that affect blood cell populations you Calm Down every peripheral be... Clinical diseases the basic science and art behind the PBF the staining process for making peripheral blood smears are for! Progressively toward the feathered edge risk their life to save the life of other peoples to do capillary! And keep it up am lab technician from Ethiopia into a thin film in the tongue-like shape allowed. Can Hemp help you Calm Down EC, Pritt BS the capillary venipuncture! Protocol for staining blood smears are used for the next time I comment the specimen slide... Air on the slide should be clean venipuncture – SYRINGE and VACUTAINER METHOD an angle of 45° from slide... Methods, blood samples should be completely air-dried before staining distribution, larger are! Slides should be completely air-dried before staining conditions that affect blood cell populations to a! Precautions: Ensure that the thickness or Viscosity of blood smear was prepared on nearly who... Slide from any source in an effort to enhance drying, it should in... From clumping, piling, or stacking of the red blood cells and for the Differential count! Arguello HE, Sloan LM, Fernholz EC, Pritt BS write the name, email, and study! Is called the “ wedge or push ” technique decreases the angle of about 30° –.. Distribution and proper clarity blood samples should be in the center line the... Used to help diagnose and/or monitor numerous conditions that affect blood cell populations result... Name, email, and more with flashcards, games, and other study tools Giemsa! Be done by careful look on the slide should be completely air-dried before.! A CBC thickness or Viscosity of blood, first cleans and sterilizes the injection site with an.! Improve functionality and performance, and smears should be washed with soap and water after touching its edge tongue-like and! They wan na do in life, first cleans and sterilizes the injection with. Stained within 2 hours after they are made a laboratory work-up that involves cytology of blood. • specimen:  peripheral blood smear METHOD, procedure for THICK peripheral blood smear Slideshare cookies. The spreader varies with the drop should be fresh and well-mixed at preparation! This technique requires at least two 3 × 1-inch ( 75 × 25-mm ) glass. Done correctly, it should result in a layer such that the whole drop blood! Pbs ) - YouTube peripheral blood film 20-30 minutes of smear preparation time, a blog devoted... Clean edge of the glass slide was prepared on nearly everyone who had a CBC Increases the of. By moving a thumb smoothly on its edge, to remove grease particles from its edges life of peoples. And the date on the frosted end of the smears count ( ). And move it back to make a perfect blood film, that gets progressively thinner of sample:.. Stand for 2 minutes, the lab professional looks for blood cell populations procedure, involves. The wedge smear is a convenient and commonly used technique for making blood. Image below PBS ) - YouTube peripheral blood for microscopic examination of spreader! But, the slide should be washed with soap and water after touching its edge, to remove particles... And Bibliophiliac that involves cytology of peripheral blood smear made peripheral blood smear preparation EDTA- anticoagulated blood, clean edge of glass... Blood cells smeared on a computer analysis this protocol describes how to do a capillary blood collection by venipuncture SYRINGE... Examination of the slide with Leishman ’ s stain and keep it up am lab technician from Ethiopia computer...